GLK-gel Affinity Chromatography Resin

Cross-linked Agarose Type

GLKgel Agarose media offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separation can often remove contaminants difficult to eliminate using conventional chromatographic procedures.

  • Uniform particle size, High resolution, Low poriness
  • High column efficiency
  • Excellent bonding and end-capping technologies
packing material for HPLC column

Main Characters

 

Pr A 4FF

Pr G 4FFIgM 6HP

IgY 6HP

Substrate

4% cross-linked agarose

6% cross-linked agarose

Ligand

rProtein A

rProtein GIgM

IgY

Particle Size

90µm (45-165µm)

37µm (25-45µm)

Capacity 

20mg hIgG/ml

25mg hIgG/ml5mg hIgG/ml

20mg hIgG/ml

pH Stability

2-10 (Short)   3-9 (Long)

2-13 (Short)   3-11 (Long)

Max. Pressure

0.3MPa

Flow Rate

300cm/h

300cm/h150cm/h

150cm/h

Storage

4-8 °C, 20% EtOH

Purification of IgG in human serum

  • Sample: 5ml human serum with five times dilution (different buffers)
  • Column: HT01 1.0ml Protein G 4FF
  • BalanceA 0.02 M PB pH7.0; B 0.02M PB, 0. 3M NaCl pH 7.0
  • Elution: 0.1 M Glycine-HCl pH2.7
  • Flow Rate: 0.25m/min (sampling), 1ml/min
agarose affinity application

Protein Purification

agarose affinity application

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