GLK-gel Affinity Chromatography Resin
Cross-linked Agarose Type
GLKgel Agarose media offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separation can often remove contaminants difficult to eliminate using conventional chromatographic procedures.
- Uniform particle size, High resolution, Low poriness
- High column efficiency
- Excellent bonding and end-capping technologies
Main Characters
Pr A 4FF | Pr G 4FF | IgM 6HP | IgY 6HP | |
Substrate | 4% cross-linked agarose | 6% cross-linked agarose | ||
Ligand | rProtein A | rProtein G | IgM | IgY |
Particle Size | 90µm (45-165µm) | 37µm (25-45µm) | ||
Capacity | 20mg hIgG/ml | 25mg hIgG/ml | 5mg hIgG/ml | 20mg hIgG/ml |
pH Stability | 2-10 (Short) 3-9 (Long) | 2-13 (Short) 3-11 (Long) | ||
Max. Pressure | 0.3MPa | |||
Flow Rate | 300cm/h | 300cm/h | 150cm/h | 150cm/h |
Storage | 4-8 °C, 20% EtOH | |||
Application
Purification of IgG in human serum
- Sample: 5ml human serum with five times dilution (different buffers)
- Column: HT01 1.0ml Protein G 4FF
- Balance：A 0.02 M PB pH7.0; B 0.02M PB, 0. 3M NaCl pH 7.0
- Elution: 0.1 M Glycine-HCl pH2.7
- Flow Rate: 0.25m/min (sampling), 1ml/min
Protein Purification
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