Liquid Chromatography Column

GALAK chromatography focus on liquid chromatography products for over 10 years. GALAK chromatography column series are versatile media based on the silica-gel/PS-DVB substrate for reversed-phase chromatography.  The spherical substrate has low metal-ion content (<20 ppm),high specific surface area and good mechanical strength. With GALAK's unique chemical bonding technique, the chromatography column series has excellent stability of bonding and end-capping. chromatography column series meet the high requirements for analysis and preparative applications.

Product Series

  1. HPLC Column
  2. Biochemical Analysis Column
  3. DNA Analysis Column >>
  4. Sugar Analysis Column >>
  5. HILIC Column >>
  6. HIC Column >>
  7. Chiral Column >>
  8. Protection/Trap Column
    • protection Column >>
    • Trap Column (Ghost remover) >>

The selection of Chromatography Column

selection of HPLC column

RPC Column Selection

UPS No.Product NameParameterFeatureDetails
L1RPC-C18MCarbone Content: 16%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Single point bonded C18 bonded silica gel column, completely end-capped
Good chromatographic performance, high column efficiency, good peak symmetry, low column loss, strong retention of hydrophobicity
Good universality with wide application
RPC Columns >>
RPC-C18HCarbone Content:20%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-11
Single point bonded C18 bonded silica gel column, completely end-capped
Good chromatographic performance, high column efficiency, good peak symmetry, low column loss, strong retention of hydrophobicity
Strong alkali resistance, higher hydrophobicity than C18M
BioC18Carbone Content: 8%
Pore Size: 300A
Specific surface area: 100m2/g
pH: 2-8
Large silica-gel particle bonded by C18 silane, completely end-capped
Excellent chromatographic performance, good selectivity, good reproducibility
Suitable for compounds with high molecular weight, such as peptides, insulin and small proteins
L7RPC-C8Carbone Content: 12%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Octylsilane bonded silica gel column, completely end-capped
Excellent chromatographic performance, high column efficiency, good peak symmetry and low column loss
Suitable for separating substances with moderate or strong hydrophobicity
BioC8Carbone Content: 5%
Pore Size: 300A
Specific surface area: 100m2/g
pH: 2-8
Alkyl silane bonded macroporous silica gel column, completelyend-capped
Excellent chromatographic performance, high column efficiency, good peak symmetry and low column loss
Suitable for compounds with higher molecular weight, such as polypeptides, insulin, and small proteins
L26RPC-C4Carbone Content: 5%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Butylsilane bonded silica gel column was completely end-capped
Excellent chromatographic performance, high column efficiency, good peak symmetry and low column loss
Suitable for the separation of highly hydrophobic substances
BioC4Carbone Content: 3%
Pore Size: 300A
Specific surface area: 100m2/g
pH: 2-8
Macroporous silica gel column bonded with butyl silane, completely end-capped
High column efficiency and peak symmetry
Suitable for the separation of high molecular weight compounds, such as polypeptides and proteins
L11PhenylCarbone Content: 8%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Phenylpropyl bonded silica gel column, completely sealed
Strong retention of aromatic compounds
Suitable for the separation of polar and aromatic compounds

NPC Column Selection

UPS No.Product NameParameterFeatureDetails
L3NPC-SilicaCarbone Content: -
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
High purity naked silica gel column
Excellent chromatographic performance, high column efficiency and peak symmetry
Suitable for the separation of fat-soluble compounds of steroids
NPC Column >>
L8NPC-NH2Carbone Content: 5%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Amino bonded silica gel column
Multiple retention mechanisms can be used in normal phase, HILIC mode, and ion exchange separation mode
Suitable for the separation of hydrophilic and polar compounds, such as carbohydrates and monosaccharides, oligosaccharides and sugar alcohols
L10NPC-CNCarbone Content: 7%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Cyano-bonded silica gel column
Weak polarity, multiple retention mechanisms of dipole-dipole action and hydrophobicity, and has unique selectivity to polar compounds
Suitable for rapid separation of hydrophobic, unsaturated, and polar compounds

HILIC Column Selection

UPS No.Product NameParameterFeatureDetails
L20HILIC-DiolCarbone Content: 10%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Diol bonded silica gel column
Good column stability and column life, medium polarity, moderate positive phase retention ability, with unique selectivity
It is suitable for the separation of hydrophilic polar compounds
HILIC Column >>
L68HILIC-AmideCarbone Content: 8%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
Acrylamide bonded silica gel column
Strong hydrogen bonding and strong selectivity to polar compounds
Suitable for the separation of small molecular polar compounds and oligosaccharides
/HILIC-ImidazoleCarbone Content: 6%
Pore Size: 120A
Specific surface area: 330m2/g
pH: 2-8
HILIC bonded silica gel column with imidazole ion
Mechanism of hydrophilic action and ion exchange mixing mode has unique selectivity
Suitable for the separation of polar compounds and negatively charged compounds

Sugar Analysis Column

UPS No.Product NameParameterFeatureDetails
L17Sugar-10HParticle Size: 6um, 8um
Degree of crosslinking: 10%
Ion exclusion chromatographic column with low crosslinking PS/DVB substrate
High resolution, good chemical stability, compatibility with 100% aqueous phase, stable batch reproducibility
Suitable for the analysis of organic acids and mixed alcohols and is also a special column for ribavirin
Sugar Analysis Column >>
L19Sugar-10CaParticle Size: 6um, 8um
Degree of crosslinking: 10%
Ca type ligand exchange column for sugar analysis of low crosslinking PS/DVB substrate
High resolution, excellent chemical stability, compatibility with 100% aqueous phase, good batch reproducibility
Suitable for the separation of monosaccharides, disaccharides, trisaccharides, tetrasaccharides, and sugar alcohols. It is also the special column for mannitol analysis
L58Sugar-10NaParticle Size: 6um, 8um
Degree of crosslinking: 10%
Na type ligand exchange column for sugar analysis of low crosslinking PS/DVB substrate
High resolution, good chemical stability, compatibility with 100% aqueous phase, good batch reproducibility
Suitable for the analysis of compounds containing large amounts of inorganic Na ions, such as honey and other oligosaccharides

Chiral Column Selection

UPS No.Product NameParameterFeatureDetails
L40Chiral ODParticle Size: 5um,10um
Pore Size: 1000A
Cellulose-tri (3, 5-dimethylphenyl carbamate) -coated silica gel chiral column
One of the most widely used high general purpose chiral chromatographic columns, especially suitable for the separation of β-blockers and steroids, such as propranolol, chlorocarbamide, flavanones, metoprolol
Chiral Column >>
L80Chiral OJParticle Size: 5um,10um
Pore Size: 1000A
Cellulose-tri (4-methylbenzoate) -coated chiral column
Suitable for ibuprofen, carbuprofen, methanone, nicotine, steroid, acetobutyl carbuprofen, albutamol, chlorocenylpropanol, mithrin, cisapride, chlorbendanol, ketamine, laudanine
 /Chiral OZParticle Size: 5um,10um
Pore Size: 1000A
Cellulose-tri (3-chloro-4-methylphenyl carbamate) - coated chiral column
Suitable for prostaglandin, o-chlormandelic acid, atenolol, chlormezazone, chloramphenyl, dipiperidon, phoxophos, nomifenoxin, phenyl succinic acid, mephentoin, laudanillin
L51Chiral ADParticle Size: 5um,10um
Pore Size: 1000A
Amylose - tri (3, 5-dimethylphenyl carbamate) - coated chiral column
One of the most widely used high-general-purpose chromatographic columns, suitable for temazepam, ephedrine, nefopam, labelol, dipepperol, cyclohexobarbital, flurbiprofen, eperisone hydrochloride, bicalutamide, approlol
L90Chiral ASParticle Size: 5um,10um
Pore Size: 1000A
Amylose - tri [(S-α-4-methylcarbamate) -coated chiral column
Suitable for o-chlormandelic acid, abscisic acid, aminoluminide, propyramide, cyclopentazide, glumide, cyclohexobarbital, imazole, ketamine, pentobarbital

Biomolecules Separation Column Selection

Column TypeProduct NameParameterFeatureDetails
Protein A Affinity ColumnAbsolut ASubstrate: PS/DVB
Ligand: rProtein A
Particle Size: 30um
Pressure: 1200psi
pH: 2-10
Direct use on HPLC instruments
High dynamic binding capacity, quick mass transfer
Minimum nonspecific absorption, accurate determination
Fast analysis cycle time: 2–5 minutes
Satisfactory linearity in wide concentration range: 0.02-10 mg/ml
Long lifetime: approx. 3000 analysis cycles
Alkali resistance: 0.1-0.5 M NaOH cleaning conditions
Absult A >>
SEC ColumnSEC-150Substrate: Monodisperse porous silica gel
Ligand: Diol
Particle Size: 5um
Pore Size: 150A
pH: 2-8
High column efficiency, good peak shape and good separation
Protein nonspecific adsorption is very low and recovery is good
Can withstand higher mechanical strength, long service life
Suitable for small molecular chemicals and polymers, polypeptides, polysaccharides, low molecular weight
Linear range of separation of oligonucleotides and proteins (globular proteins) : 5,000-150,000Da
SEC Column >>
SEC-300Substrate: Monodisperse porous silica gel
Ligand: Diol
Particle Size: 5um
Pore Size: 300A
pH: 2-8
High column efficiency, good peak shape and good separation
Protein nonspecific adsorption is very low and recovery is good
Can withstand higher mechanical strength, long service life
Linear range (globular protein) for separation of monoclonal antibodies and polymers: 10,000-1,000,00 Da
SEC-500Substrate: Monodisperse porous silica gel
Ligand: Diol
Particle Size: 5um
Pore Size: 500A
pH: 2-8
High column efficiency, good peak shape and good separation
Protein nonspecific adsorption is very low and recovery is good
Can withstand higher mechanical strength, long service life
Linear range for separation of high molecular weight proteins and polymers (globular proteins) : 20,000-2,000,000Da
HIC ColumnHIC-ButylSubstrate: Monodisperse porous silica gel
Ligand: Butyl
Particle Size: 5um
Pore Size: 1000A
pH: 2-8
The unique chemical design provides excellent selectivity for monoclonal antibody conjugated drug molecules (ADCs)
Ultra-large pore mono-disperse silica gel base sphere, high column efficiency, low nonspecific adsorption, high recovery
Good resistance to pressure and organic solvents. Good consistency between lots
Suitable for the separation and characterization of monoclonal antibody and monoclonal antibody conjugated drug molecules
HIC Column >>

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