Gel Filtration Chromatography Media

Gel filtration chromatography achieves the purpose of separation according to the size of solute molecules. Gel filtration chromatography is also known as volume exclusion chromatography, molecular sieve chromatography. Gel filtration chromatography media are inert spherical granular materials with a porous network structure.

GALAK provides two types of gel filtration media: crosslinked agarose microspheres and crosslinked glucan microspheres.

Gel Filtration Chromatography

GLKgel GFC Agarose Media

GALAK has two types of agarose gel filtration media: 4% and 6% agarose gel. Crosslinked agarose gel (CL) is a low-strength crosslinking agarose gel with better physical and chemical stability. High-velocity agarose gel (FF) is high strength crosslinking agarose gel with good physical and chemical stability, can be sterilized by heat and humidity, and withstand various working conditions in protein production.

ProductsMolecular Weight (globulin)Particle SizePressureFlow RatepH Range
GFC 4B6×104-2×10790μm (45-165μm)

 

Customized Size:

25-46μm & 165-300μm

≤0.02MPa12 cm/h4-9 (Long) 4-9 (Short)
GFC 4FF7×104-2×107≤0.3MPa250 cm/h2-12 (Long) 2-14 (Short)
GFC 6B1×104-4×106≤0.02MPa14 cm/h4-9 (Long) 4-9 (Short)
GFC CL-6B1×104-4×106≤0.05MPa30 cm/h3-12 (Long) 2-14 (Short)
GFC 6FF1×104-4×106≤0.3MPa300 cm/h2-12 (Long) 2-14 (Short)

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GLKgel GFC Glucan Media

Cross-linked glucan is a globular gel that contains a large number of hydroxyl groups and is prone to swelling in water and electrolyte solutions. G-type cross-linked glucans have different crosslinking degrees, their swelling degree and separation range are different. The swelling degree of crosslinked glucan is not affected by the presence of salt and detergent.

ProductsMolecular Weight (globulin)Particle SizeSwelling DegreeFlow RatepH Range
GFC G-25 Coarse1×103-5×103165-300μm4-6 ml/g300 cm/h2-13
GFC G-2545-165μm4-6 ml/g150 cm/h
GFC G-753×103-8×10445-165μm12-15 ml/g80 cm/h
GFC G-75 Fine25-45μm12-15 ml/g20 cm/h

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