Gel Filtration Chromatography Media
Gel filtration chromatography achieves the purpose of separation according to the size of solute molecules. Gel filtration chromatography is also known as volume exclusion chromatography, molecular sieve chromatography. Gel filtration chromatography media are inert spherical granular materials with a porous network structure.
GALAK provides two types of gel filtration media: crosslinked agarose microspheres and crosslinked glucan microspheres.
GLKgel GFC Agarose Media
GALAK has two types of agarose gel filtration media: 4% and 6% agarose gel. Crosslinked agarose gel (CL) is a low-strength crosslinking agarose gel with better physical and chemical stability. High-velocity agarose gel (FF) is high strength crosslinking agarose gel with good physical and chemical stability, can be sterilized by heat and humidity, and withstand various working conditions in protein production.
|Products||Molecular Weight (globulin)||Particle Size||Pressure||Flow Rate||pH Range|
|GFC 4B||6×104-2×107||90μm (45-165μm)|
25-46μm & 165-300μm
|≤0.02MPa||12 cm/h||4-9 (Long) 4-9 (Short)|
|GFC 4FF||7×104-2×107||≤0.3MPa||250 cm/h||2-12 (Long) 2-14 (Short)|
|GFC 6B||1×104-4×106||≤0.02MPa||14 cm/h||4-9 (Long) 4-9 (Short)|
|GFC CL-6B||1×104-4×106||≤0.05MPa||30 cm/h||3-12 (Long) 2-14 (Short)|
|GFC 6FF||1×104-4×106||≤0.3MPa||300 cm/h||2-12 (Long) 2-14 (Short)|
GLKgel GFC Glucan Media
Cross-linked glucan is a globular gel that contains a large number of hydroxyl groups and is prone to swelling in water and electrolyte solutions. G-type cross-linked glucans have different crosslinking degrees, their swelling degree and separation range are different. The swelling degree of crosslinked glucan is not affected by the presence of salt and detergent.
|Products||Molecular Weight (globulin)||Particle Size||Swelling Degree||Flow Rate||pH Range|
|GFC G-25 Coarse||1×103-5×103||165-300μm||4-6 ml/g||300 cm/h||2-13|
|GFC G-25||45-165μm||4-6 ml/g||150 cm/h|
|GFC G-75||3×103-8×104||45-165μm||12-15 ml/g||80 cm/h|
|GFC G-75 Fine||25-45μm||12-15 ml/g||20 cm/h|