HILIC Column

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic technique used to improve the retention of strong polar substances in reversed-phase chromatography. It works by adopting a strong polar stationary phase and combining the mobile phase with a high proportion of organic phases. HILIC has a wide variety of stationary phases, and in principle, any stationary phase with the polar surface can use in HILIC mode. Therefore, stationary phases such as silica, amino (NH2), diol, and cyanogen (CN) in the positive phase chromatography can also use as stationary phases for HILIC.

Galaksil® HILIC-Diol

Particle Size Pore Size Surface Area Carbon Content pH Range
3/5/10um120Å330m2/g10%2-8

Galaksil® HILIC-Amide

Particle Size Pore Size Surface Area Carbon Content pH Range
3/5/10um120Å330m2/g8%2-8

Galaksil® HILIC-Imidazole

Particle Size

Pore Size Surface Area Carbon Content pH Range
3/5/10um120Å330m2/g6%

2-8

Application

hilic diol column

Column: HILIC-Diol 5 μm

Dimension: 4.6×250mm

Mobile phase:

Blue: 90/10 v/v MeCN /100 mM HCOONH4 (pH3.6)

Black: 90/10 v/v MeCN /100 mM HCOONH4 (pH5.2)

Flow rate: 1 mL/min

Temperature: 30℃

Injection: 5 μL

Detection: 218 nm

Peaks: 1. DICY; 2. MET; 3. Melamine.

hilic amide column

Column: HILIC-Amide 5 μm

Dimension: 4.6×250mm

Mobile phase:

80/10 v/v MeCN /100 mM CH3COOH

Flow rate: 0.5 mL/min

Temperature: 20℃

Injection: 10 μL

Detection: CDA

Peaks: 1. Stachydrine

 

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