HPLC Column Selection By USP Specification

The following list of USP (United States Pharmacopoeia) column specifications (USP 35) includes a selection of recommended columns within each category. In most cases, there are several columns available within a given category, but in a few indicated instances a packing very closely fitting the specification has been included. Please contact us for further advice and assistance on selecting a suitable column by USP specification. Please also contact us for advice on column selection by EP (European Pharmacopoeia) specification. The USP monographs allow chromatographers flexibility to make method adjustments within specified limits in order to meet system suitability requirements.

L1Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10μm in diameter, or a monolithic rodGALAK ODS Column
L2Octadecylsilane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50μm in diameterGALAK C18 Column
L3Porous silica particles, 1.5 to 10μm in diameter, or a monolithic silica rodGALAK Silica Column
L4Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50μm in diameterGALAK Silica Column
L5Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50μm in diameter
L6Strong cation-exchange packing – sulphonated fluorocarbon polymer coated on a solid spherical core, 30 to 50μm in diameter
L7Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10μm in diameter, or a monolithic silica rodGALAK C8 Column
L8An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10μm in diameterGALAK NH2 Column
L9Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10μm in diameter
L10Nitrile groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterGALAK CN Column
L11Phenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterGALAK Phenyl Column
L12A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50μm in diameter
L13Trimethylsilane chemically bonded to porous silica particles, 3 to 10μm in diameter
L14Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10μm in diameter
L15Hexylsilane chemically bonded to totally porous silica particles, 3 to 10μm in diameter
L16Dimethylsilane chemically bonded to porous silica particles, 5 to 10μm in diameter
L17Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12μm in diameter
L18Amino and cyano groups chemically bonded to porous silica particles, 3 to 10μm in diameter
L19Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9μm in diameter
L20Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10μm in diameterGALAK Diol Column
L21A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30μm in diameter
L22A cation-exchange resin made of porous polystyrene gel with sulphonic acid groups, about 10μm in size
L23An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, 7 to 12μm in size
L24Polyvinyl alcohol chemically bonded to porous silica particles, 5μm in diameter
L25Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable
L26Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameterGALAK C4 Column
L27Porous silica particles, 30 to 50μm in diameter
L28A multifunctional support, which consists of a high purity, 100A, spherical silica substrate that has been bonded with anionic exchanger, amine functionality in addition to a conventional reversed-phase C8 functionality
L29Gamma alumina, reversed-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5μm in diameter with a pore volume of 80A units
L30Ethylsilane chemically bonded to totally porous silica particles, 3 to 10μm in diameter
L31A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5μm macroporous particles having a pore size of 2000A units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene
L32A chiral ligand-exchange resin packing - L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10μm in diameter
L33Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability
L34Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 7 to 9μm in diameter
L35A zirconium-stabilised spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150A
L36A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5μm aminopropyl silica
L37Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a polymethacrylate gel
L38A methacrylate-based size-exclusion packing for water-soluble samples
L39A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin
L40Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5μm to 20μm in diameter
L41Immobilised α1-acid glycoprotein on spherical silica particles, 5μm in diameterGALAK Chrial OD-H
L42Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5μm in diameter
L43Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10μm in diameter
L44A multifunctional support, which consists of a high purity, 60A, spherical silica substrate that has been bonded with a cationic exchanger, sulphonic acid functionality in addition to a conventional reversed phase C8 functionality
L45Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles, 5 to 10μm in diameter
L46Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalised latex beads, about 9μm to 11μm in diameter
L47High capacity anion-exchange microporous substrate, fully functionalised with a trimethylamine group, 8μm in diameter
L48Sulphonated, cross-linked polystyrene with an outer layer of sub-micron, porous, anion-exchange microbeads, 5 to 15μm in diameter
L49A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 to 10μm in diameter
L50Multi-function resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15μm in diameter, and a surface area of not less than 350 m2/g. Substrate is coated with quaternary ammonium functionalised latex particles consisting of styrene cross-linked with divinylbenzene
L51Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 10μm in diameter
L52A strong cation-exchange resin made of porous silica with sulphopropyl groups, 5 to 10μm in diameter
L53Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15μm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalised monomers. Capacity not less than 500 μEq/column
L54A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13μm in diameter
L55A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5μm in diameter
L56Propyl silane chemically bonded to totally porous silica particles, 3 to 10μm in diameter
L57A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5μm in diameter, with a pore size of 120A
L58Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30μm in diameter
L59

 

Packing for the size exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. It is spherical (1.5 to 10μm), silica or hybrid packing with a hydrophilic coating
L60Spherical, porous silica gel, 10μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and endcapped
L61A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13μm microporous particles having a pore size less than 10A units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%)
L62C30 silane bonded phase on a fully porous spherical silica, 3 to 15μm in diameter.
L63Glycopeptide teicoplanin linked through multiple covalent bonds to a 100A units spherical silica
L64Strongly basic anion-exchange resin consisting of 8% crosslinked styrene-divinylbenzene copolymer with a quaternary ammonium group in the chloride form, 45 to 180μm in diameter
L65Strongly acidic cation-exchange resin consisting of 8% sulphonated crosslinked styrene-divinylbenzene copolymer with a sulphonic acid group in the hydrogen form, 63 to 250μm in diameter
L66A crown ether coated on a 5μm particle size silica gel substrate. The active site is (S)-18-crown-6-ether
L67Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10μm in diameter
L68Spherical, porous silica, 10μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped
L69Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalised 130nm latex beads, about 6.5μm in diameter
L70Cellulose tris(phenyl carbamate) coated on 5μm silica
L71A rigid, spherical polymethacrylate, 4 to 6μm in diameter
L72(S)-phenylglycine and 3,5-dinitroaniline urea linkage covalently bonded to silica
L73A rigid, spherical polydivinylbenzene particle, 5 to 10μm in diameter
L74A strong anion-exchange resin consisting of a highly cross-linked core of 7μm macroporous particles having a 100A average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions
L75A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7μm in diameter, with a pore size of 300A
L76Silica based weak cation-exchange material, 5μm in diameter. Substrate is surface polymerised polybutadiene-maleic acid to provide carboxylic acid functionalities. Capacity not less than 29 μEq/column
L77Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to 9μm diameter. Substrate is surface grafted with carboxylic acid functionalised groups. Capacity not less than 500 μEq/column (4mm x 25cm)
L78A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, tertiary or quaternary amino) functional groups chemically bonded to porous, non-porous or ceramic microparticles, 1.0 to 50μm in diameter or a monolithic rod
L79A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5μm in diameter
L80Cellulose tris(4-methylbenzoate)-coated, porous spherical silica particles, 5μm in diameter
L81A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9μm porous particles having a pore size of 2000A and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 70nm diameter microbeads (6% cross-linked) bonded with alkanol quaternary ammonium ions
L82Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4-5μm in diameter
L83A hydroxide-selective, strong anion-exchange resin, quaternary amine bonded on latex particles attached to a core of 10.5μm microporous particles having a pore size of 10A and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene
L84Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5μm diameter. Substrate is surface grafted with carboxylic acid functionalised groups. Capacity not less than 8400 μEq/column (5mm x 25cm)

USP and EP Allowable Adjustments

ParameterUSP Allowable Adjustment *EP Allowable Adjustment *
Column Length±70%±70%
Column i.d.±25%±25%
Particle Size–50%–50%
Column Temperature±10℃±10℃
Flow Rate±50%±50%
Eluent pH±0.2 units±0.2 units
Concentration of Buffer Salts±10%±10%
Solvent A: B RatioMinor ±30% relative, but±10% absoluteMinor ±30% relative or ±2% absolute, ≤±10% absolute for other
Injection VolumeAny reductionAny reduction
Change of UV Detector Wavelength0, but ±3nm between detectorsNo change

* United States Pharmacopoeia 34 (2011) Section 621; European Pharmacopoeia 6.0 (2010) Section 2.2.4.6

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