Ion-exchange Agarose Resin

Based on agarose microspheres, GLKgel IEX resin provide SP, Q, ANX, DEDA, MMC, MMA series of ion-exchange chromatography media to need different purification processes.

ion-exchange chromatography media

GLKgel Strong Cation IEX SP Resin

  SP 6BB SP 6FF SP 6HF SP 6HP SP 6XL SP HPR
Substrate 6% cross-linked Agarose High-rigid Agarose 6% cross-linked Agarose 6% cross-linked Agarose with glucan High-rigid Agarose
Particle Size 200μm

(165-300μm)

90μm

(45-165μm)

90μm

(45-165μm)

37μm

(25-45μm)

90μm

(45-165μm)

37μm

(25-45μm)

Ligand -CH2CH2CH2SO3-
Loading

Capacity

180-250μmol

H+/ml resin

140-200μmol H+/ml resin 150-200μmol H+/ml resin 180-250μmol H+/ml resin 130-160μmol H+/ml resin
pH Stability 4-13 (Long)

3-14 (Short)

4-12 (Long)

3-14 (Short)

4-13 (Long)

3-14 (Short)

4-12 (Long)

3-14 (Short)

Pressure ≤0.3MPa ≤0.5MPa
Flow Rate 1800cm/h 700 cm/h 100 cm/h 150 cm/h 700 cm/h 400 cm/h
Chemical Stability All common buffer, 1.0m sodium hydroxide, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage 0.2M NaAc, 20% EtOH, 4-30℃

GLKgel Strong Anion IEX Q Resin

  Q 6BB Q 6FF Q 6HF Q 6HP Q 6XL Q HPR
Substrate 6% cross-linked Agarose High-rigid Agarose 6% cross-linked Agarose 6% cross-linked Agarose with glucan High-rigid Agarose
Particle Size 200μm

(165-300μm)

90μm

(45-165μm)

90μm

(45-165μm)

37μm

(25-45μm)

90μm

(45-165μm)

37μm

(25-45μm)

Ligand -N+(CH3)3
Loading Capacity 180-250μmol

Cl-/ml resin

160-200μmol

Cl-/ml resin

140-200μmol

Cl-/ml resin

180-250μmol Cl-/ml resin 150-180μmol Cl-/ml resin
pH Stability 2-12 (Long Period)

2-14 (Short Period)

2-12 (Long)

2-14 (Short )

2-12 (Long)

2-14 (Short)

2-12 (Long )

2-14 (Short)

Pressure ≤0.3MPa ≤0.5MPa
Flow Rate 1800cm/h 700 cm/h 1000 cm/h 150 cm/h 700 cm/h 400 cm/h
Chemical Stability

All common buffer, 1.0m sodium hydroxide, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage 20% EtOH, 4-30℃

GLKgel Strong Anion IEX MMA Resin

Substrate Particle Size Capacity pH Stability Pressure

Flow Rate

MMA 6HF

High Rigid

Agarose

90μm

(45-165μm)

90-120μmol

Cl-/ml resin

2-14 (Long)

4-12 (Short)

≤0.5 MPa 1000 cm/h
MMA HPR High Rigid

Agarose

37μm

(25-45μm)

80-110μmol

Cl-/ml resin

2-14 (Long)

4-12 (Short)

≤0.5 MPa

400 cm/h

Chemical Stability

All common buffer, 1.0m NaOH, 8.0m urea, 6.0m GuHCl, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage

  20% EtOH, 4-30℃

GLKgel Weak Cation IEX CM Resin

  CM 6FF CM 6HF CM 6HP CM 6XL
Substrate 6% cross-linked

Agarose

High-rigid

Agarose

6% cross-linked

Agarose

6% cross-linked Agarose with glucan
Particle Size 90μm (45-165μm) 90μm (45-165μm) 37μm (25-45μm) 90μm (45-165μm)
Ligand -O-CH2COO-
Capacity 90-130μmol

H+/ml resin

90-120μmol

H+/ml resin

80-110μmol

H+/ml resin

180-250μmol

H+/ml resin

pH Stability 4-13 (Long)

2-14 (Short)

4-12 (Long)

3-14 (Short)

4-13 (Long)

2-14 (Short)

Pressure ≤0.3 MPa ≤0.5MPa ≤0.3 MPa
Flow Rate 700 cm/h 1000 cm/h 150 cm/h 700 cm/h

Chemical

Stability

All common buffer, 1.0m NaOH, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage  20% EtOH, 4-30℃

GLKgel Weak Cation IEX MMC Resin

 

Substrate Particle Size Capacity pH Stability pH Stability Flow Rate
MMC 6HF High Rigid

Agarose

90μm

(45-165μm)

70-90μmol

H+/ml resin

2-14 (Long)

3-12 (Short)

≤0.5 MPa

1000 cm/h

MMC HPR

High Rigid

Agarose

37μm

(25-45μm)

60-80μmol

H+/ml resin

2-14 (Long)

3-12 (Short)

≤0.5 MPa 400 cm/h

Chemical Stability

All common buffer, 1.0m NaOH, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage

  20% EtOH, 4-30d

GLKgel Weak Anion IEX DEDA Resin

  DEDA 6FF DEDA 6HF DEDA 6HP DEDA 6XL
Substrate 6% cross-linked Agarose High-rigid Agarose 6% cross-linked Agarose 6% cross-linked Agarose
Particle Size 90μm (45-165μm) 90μm (45-165μm) 37μm (25-45μm) 90μm (45-165μm)
Ligand -N+(CH3)3
Capacity 110-160μmol

Cl-/ml resin

290-350μmol

Cl-/ml resin

90-130μmol

Cl-/ml resin

110-160μmol

Cl-/ml resin

pH Stability 2-13 (Long)

1-14 (Short)

2-12 (Long)

2-14 (Short)

2-13 (Long)

1-14 (Short)

Pressure ≤0.3 MPa ≤0.5MPa ≤0.3 MPa
Flow Rate 700 cm/h 1000 cm/h 150 cm/h 700 cm/h
Chemical Stability  All common buffer, 1.0m NaOH, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage   20% EtOH, 4-30℃

GLKgel Weak Anion IEX ANX Resin

  Substrate Particle Size Ligand Capacity pH Stability pH Stability Flow Rate
ANX 4FF 4% cross-linked Agarose 90μm (45-165μm) -N+(C2H5)2H 130-170μmol

Cl-/ml resin

3-10 (Long)

2-14 (Short)

≤0.3 MPa 250 cm/h
Chemical Stability

All common buffer, 1.0m NaOH, 8.0m urea, 6.0m guanidine hydrochloride, 70% ethanol

Avoid using oxidant, cationic detergent, cationic buffer

Storage   20% EtOH, 4-30℃

Related Products

Sepromax PS/DVB Resin

  • S40 Strong Cation Exchange Media
  • CM40 Weak Cation Exchange Media
  • Q40 Strong Anion Exchange Media
  • D40 Weak Anion Exchange Media

Glass Column

  • Fixed-bed glass column
  • Single-layer glass column
  • Double-layer glass column

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