mAb Analysis HPLC Column

Protein A Affinity Column Absolut A

mAb analysis HPLC column is used for monoclonal antibody analysis in labs. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Protein A affinity chromatography is a fast, accurate, and reliable method. Absolut A is protein A affinity columns for mab analysis.

  • Direct use on HPLC instrument
  • Fast and accurate analysis of antibody concentration
  • Wide linearity range
Protein A affinity Chromatography HPLC Column mab antibody analysis

mAb Analysis HPLC Column Technical Parameters

 LigandSubstratePressurepH RangeDynamic CapacityFlow RateLife Time
Absolut ArProtein A30 μm PSDVB1200psi2-1140mg human IgG/ml media1-3ml/min3000 circles
Absolut A+rProtein A30 μm PSDVB1200psi2-1140mg human IgG/ml media1-3ml/min5000 circles
  • Lifetime is based on laboratory tests. Actual data is according to sample properties.
  • PS/DVB: Poly(styrene/divinylbenzene)

Advantages of Absolut A

  • Mono-dispersed PS/DVB particles feature very high rigidity, low back pressure, and quick mass transfer.
  • Unique hydrophilic surface treatment technology minimizes the nonspecific absorption and guarantees accurate determination of antibody concentration.
  • The protein binds specifically to the Fc region of IgG, and the high selectivity guarantees the accuracy of concentration determination.
  • Fast analysis cycle time: 2 – 5 minutes.
  • Satisfactory linearity within a wide concentration range from 0.02 to 10 mg/ml.
  • Long lifetime: 3000-5000 analysis cycles.
  • New alkali-resistant Protein A ligand warrants 0.1-0.5M NaOH cleaning conditions.

Performance Testing For mAb Analysis HPLC Column

1. Excellent Linearity

Testing process for Absolut A
Standard curves for Absolut A

2.Flexible choice of flow rate

flexiable choice of flow rate mAb Analysis HPLC Column

3.Lifetime testing

life time test for Absolut A mAb Analysis HPLC Column

Application

(1) Fc Fusion Protein

(2) Monoclonal antibody

absolut a application image
absolut a application image
absolut a application image
absolut a application image

Analysis Condition Of mAb Analysis HPLC Column

Eluent A20-50 mM Phosphate, 150 mM NaCl, pH7-7.5
Eluent B12 mM HCl, 150 mM NaCl, pH1.9, or 100 mM Gly-HCl, 150 mM NaCl, pH2.5
Flow rate1 mL/min
Injection1-100 μL
DetectionUV 280nm

Method Timetable

Time(min)Gradient(%B)
0.000Equilibration/Wash, 1 min, 10 CV
1.000Equilibration/Wash, 1 min, 10 CV
1.01100Elution, 2 min, 20 CV
3.00100Elution, 2 min, 20 CV
3.010Reequilibration, 2 min, 20 CV
5.000Reequilibration, 2 min, 20 CV

* the analysis condition varies according to different machines.

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