GLK-gel Ni Affinity Resin

GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium,which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.

  • Ni NTA/NTA+ Resin
  • Ni IDA Resin
  • Ni TED Resin
  • Benzamidine Affinity Media
Nickel affinity resin

GLK-gel Ni NTA/NTA+ Resin

GLK gel Ni NTA/NTA+ media use Ni2+ to interact with amino acids (histidine, cysteine, tryptophan) on the side chain of the protein. It is suitable for the separation and purification of His tag proteins and biomolecules interacting with Ni2+.

Substrate6% high cross-linked agarose
Particle Size90µm (45-165μm) GLK Ni NTA; 37μm(25-45μm) GLK Ni NTA+
Binding Capacity Approx. 40 mg His (tag protein)/ml media
pH Stability3-12 (Working); 2-14 (Cleaning)
Max. Pressure300 bar (43.5 psi)
Chemical Stability0.01M HCl; 0.01M NaOH (one week);1M NaOH; 70%EtOH(12 hours); 2% SDS(1 hour); 30% isopropanol (0.5 hour)
Storage4-15 °C, 20℃ EtOH

GLK-gel Ni IDA Resin

Substrate6% high cross-linked agarose
Particle Size90µm (45-165μm)
Binding Capacity Approx. 45 mg His (tag protein)/ml media
pH Stability3-12 (Working); 2-14 (Cleaning)
Max. Pressure300 bar (43.5 psi)
Chemical StabilityCommon aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE)
Storage4-15 °C, 20℃ EtOH

GLK-gel Ni TED Resin

Substrate6% high cross-linked agarose
Particle Size90µm (45-165μm)
Binding Capacity Approx. 20 mg His (tag protein)/ml media
pH Stability3-12 (Working); 2-14 (Cleaning)
Max. Pressure300 bar (43.5 psi)
Chemical Stability0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min)
Storage4-15 °C, 20℃ EtOH

GLKgel Benzamidine Affinity Media

 Benzamidine 4FF-HSBenzamidine 6FF
Substrate4% cross-linked agarose6% agarose
LigandPara amphenamidinePara amphenamidine
Particle Size90µm (45-165µm)
Capacity 35mg trypsin /ml13mg trypsin/ml
pH Stability1-9 (Short)   2-8 (Long)
Max. Pressure0.3MPa0.02MPa
Flow Rate300cm/h300cm/h
Storage4-8 °C, 0.05M acetate buffer, 20% EtOH , pH4.0

Ni Affinity Resin Application

Ni NTA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

Ni IDA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

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