Ni NTA Magnetic Beads

Ni NTA magnetic beads design for simple small-scale purification of histidine-tagged proteins. Magarose beads are suitable for purification of a single sample or multiple samples in parallel for example in screening experiments.

GALAK Magarose Beads can be used together with Eppendorf microcentrifuge tubes and a magnetic rack, for example, MagRack 6. GALAK Magarose Beads are easily separated from the liquid phase during the different steps of the purification protocol.

Ni-NTA-Magarose-Beads

Ni NTA Magnetic Beads Parameters

SubstrateMagnetic agarose microspheres
Combined ability (/ml magnetic taste)>10mg 6XHis-tagged protein
Particle size30-100 μm
Storage buffer1XPBS containing 20% ethanol
VolumeSuspend in protection solution, 20% content
Protective buffer20% EtOH 1XPBS
Storage temperature2°C-8°C

Ni NTA Magnetic Beads Introduction

Ni NTA Magarose Beads is designed for efficient/protein purification histidine labels quickly and design of new products, magnetic microspheres can step in under the action of magnetic field directly from biological samples purify high purity of target protein, greatly simplifies the purification technology and purification efficiency, suitable for scientific research and industrial customers high flux to histidine label protein purification.

Compared with the traditional column chromatography method, Ni NTA Magarose Beads do not need to control the flow rate of sample preparation, or expensive chromatography and centrifugation equipment. The combination of samples and magnetic Beads and the separation of target proteins and magnetic Beads become very simple and fast, and it is easier to achieve a high-throughput, automated protein purification method.

Ni NTA Magarose Beads are based on 4% agarose gel as matrix. Through chemical coupling of tetrachonitric triacetic acid (NTA) and chelating nickel ions (N2+), form a very stable octahedral structure. Nickel ions are in the center of the octahedron, and nickel ions are more stable.

Ni NTA magnetic beads

Reagent Tolerance of Ni NTA Magarose Beads

ReagentsConcentration
Reducing agent5 mM DTE

1mM DTT

20 mM β-mercaptoethanol

5 mM TCEP

10mM reduced glutathione

Denaturing reagent8 M urea

6 M Gua-HCI

Abluent2% TritonTM X-100 (nonionic)

2% TweenTM 20 (nonionic)

2% NP-40 (nonionic)

2% cholate (anionic)

1% CHAPS (zwitterionic)

Other reagents500 mM imidazole

20% ethanol

50% glycerol

100 mM Na2SO4

1.5 M NaCl

1 mM EDTA

60 mM citrate

Buffer50 mM sodium phosphate, pH 7.4

100 mM Tris-HCl, pH 7.4

100 mM Tris-acetate, pH7.4

100 mM HEPES, pH 7.4

100 mM MOPS, pH 7.4100 mM sodium acetate pH 4

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