Perfusion Chromatography Media
Perfusion chromatography is a technique that arises to overcome the problem associated with mass transfer in the separation of large molecules such as proteins by liquid chromatography. Product production began as early as 1990s. Perfusion media are based on high crosslinked polystyrene divinylbenzene (PS-DVB) with two sets of pores: throughpores (6000-8000 Å) and diffusive pores (800-1500 Å). Throughpores enable better access of macromolecules to the inner of the particle, and increase the mobile phase velocity without any reduction of the column separation. With a structure of rich diffusion holes, the specific surface area of perfusion media is not reduced due to the presence of thoughpores. The dynamic capability of the perfusion chromatography media is stable with the increase of the flow rate.
Perfusion chromatography is an ideal choice the separation and purification of biological macromolecules widely used in ion-exchange, affinity, and hydrophobic interactive chromatography. They have excellent performance for complex biological samples with high viscosity, large molecule, high mass transfer barrier, and unstable biomolecules. The safety and reliability of perfusion chromatography technology have been proven in the production of FDA-approved biotherapeutic products.
Tradition Liquid Chromatography Media
GALAK Perfusion Chromatography Media
VirCap® Perfusion Chromatography Media
Combining innovative bonding and surface treatment techniques, GALAK Chromatography developed series of high-performance VirCap® perfusion chromatography media. VirCap® is hydrophilic porous polymer microspheres developed based on PS-DVB microspheres with extremely low non-specific adsorption. VirCap® media are top-performing chromatographic media for process-scale bioseparations. The rigid, robust particles enable high-resolution separations with 2–3x the throughput of conventional fast-flow gels. They are easier to handle and pack and offer outstanding cleanability.
VirCap® perfusion chromatography media offer better choices for your purification processes in biological science, biological engineering and pharmaceutical industry areas.
Ion-exchange, affinity and hydrophobic interactive media. Multiple choices for different application.
Rigid Microsphere With Large Pore Size
The core of VirCap® particles is the novel porous structure with large “throughpores”. These large throughpores allow part of mobile phase flow through, quickly carrying biomolecules to smaller diffusive pores. The large “throuhpores” reduce diffusion rate of biomolecules and enhance interaction between biomolecules and functional groups on the surface. Consequently, diffusion barriers are lowered, and flow rate can be dramatically increased-without compromising capacity or resolution.
- 3000-6000Å pore size, enable the diffusion and mass transfer for large biomolecules.
- 45-90 micron particles, satisfy your purification processing requests.
Flexible tentacle structure minimizes the steric hindrance between functional groups and target molecules. It also improves the binding capability of the target material.
Compared to traditional media, VirCap® media have more effective capture and higher total recovery.
High Flow Rate, Low Backpressure
VirCap® media offer an excellent balance of resolution and operating backpressure.
Under the condition of mobile phase change, VirCap® media exhibites almost no shrinkage or expansion. Throughpores and flexible tentacles ensures the rapid diffusion of solute, reduces the barrier of mass transfer, and realizes the high dynamic load under the operation of high flow rate.
Robust Chemical Stability
VirCap® media are rigid polymeric particles coated with a proprietary hydrophilic polymer onto which the various functional groups (ion exchange, affinity, etc.) are covalently attached. The result is a highly robust, chemically stable product that is ideally suited for large-scale biopharmaceutical applications.
VirCap® AF Perfusion Chromatography Media
Virus purification is the basis of developing high-quality vaccine, and also provides an important basis for the study of virus fine morphological structure, isolation and purification of virus antigen protein, and detailed studies of virus chemical composition and genetic material.
In traditional virus vaccine purification processing, the sucrose density gradient ultrafast purification process requires high-cost large centrifugal devices. These devices with small sample loading, long treatment time, and poor removal of host cell DNA. Gel filtration chromatography, widely used in a vaccine purification process, is mild, repeatable and easy to be industrialized. But its efficiency is low, and the effect of remove heteroprotein and DNA is not obvious.
VirCap® AF media is an affinity chromatography media designed for the capture and moderate purification stages of capsular virus purification. Specific adsorption of VirCap® AF media and target occurs by simulating the affinity between ligands and virus particles with capsular membranes. With unique high loading capacity, high flow rate and low back pres-sure, VirCap® AF media reduces the process cycle time and increases the yield, fully meeting the requirements of large-scale biopharmaceutical and vaccine production processes.
VirCap® AF Media Advantages:
√ High selectivity, similar affinity to live and inactivated capsular viruses.
√ Closed operation, safety, and sterility.
√ Enrichment and purification are carried out simultaneously, increase production, reduce processing steps, time and cost.
√ Rigid particles can be operated under a high flow rate, improve the purification efficiency.
√ Good chemical stability, longer service life.
VirCap® AF Media Parameter
|Substrate||Hydrophilic PS/DVB Microspheres|
|Dynamic Binding Capability||100mg lysozyme/ml|
(20℃, buffer solution viscosity same as water，pressure < 3 bar / 43.5psi, column bed height 20cm)
|Column Bed Height||20-40cm|
|CIP Condition||0.5-1M NaOH|
|Storage||2-8℃ 20% EtOH|
VirCap® AF Media Application
|Rabies||Feline Calicivirus||Herpes Simplex gA and gB Glycoprotein Subunits|
|Influenza||Respiratory Syncytial Virus||Hepatitis B Surface Antigen|
|Japanese Enchephalitis||Human Herpes Simplex||Filamentous Hemagglutinin from B. pertussis|
|Feline Leukemia||Human Measles||Leucocytosis Promoting Factor Hemagglutinin|
|Feline Herpes||Human Parainfluenza|
GALAK Technical Service
Analysis & Research
- Sample Testing
- Select Chromatography Media
- Select purification condition
- Optimum technology
- Support devices
- Media and process conditions
- Complete technical development
- Local installation
- Staff training
- Device debugging
- Pilot production