Protein A Affinity Resin
Sepromax® A50
Sepromax® A50 is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Sepromax® A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).
The ligand of Sepromax® A50 is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS/DVB microsphere substrate. The rProtein A has a better alkali-resisting ability that ensures stability in high pH conditions. With our hydrophilic treatment and binding technology, we eliminated non-specific binding PS-DVB surface. Hence, Sepromax® A50 is extremely useful for the purification process of monoclonal antibodies.
Advantages：
- High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing
- Outstanding high dynamic binding capacity at low residence time reduces process time and the amount of medium used
- Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP
- Low ligand leakage ensures a long lifetime
- High price-performance ratio
Main Characteristics
| Support Matrix | Poly(styrene/divinylbenzene) (PS-DVB) |
| Ligand | Recombinant Protein A |
| Ave. Particle size | 50µm |
| Dynamic Binding Capacity (DBC) | Approx. 40 mg human IgG/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 5 cm, Residence time 0.6 min) |
| Shrinkage/Swelling | < 1% from 1-100% organic solvent |
| pH range (Long term) | pH 2-10 |
| Maximum Operating Pressure | 1500 psi (100 bar / 10 MPa) |
| Cleaning Agents | 0.1-0.5M NaOH |
| Temperature Stability | 4-40 °C |
| Delivery Conditions | 20% ethanol (2-8℃) |
Performance Testing
1. Particle images
2. Dynamic Binding Capacity (DBC)
| Test Condition | |
|---|---|
| System | AKTA® Purifier10 |
| Initial Buffer | 20 mM PB，0.15 M NaCl，pH 7.4 |
| Elution Buffer | 0.1 M Gly-HCl, pH 2.5 |
| CIP solution | 0.5 M NaOH |
| Sample | 1.0 mg/ml hIgG |
3. Protein A Ligand Leakage Test (ELISA)
Samples: 20mg hlgG/ml-resin, 9mg mAb/ml-resin
ELISA test: Cygnus F400 kit
4. Alkali Resistance Test (150 Cycle Lifetime)
Protein A Affinity Resin Application
1.Fc Protein
2.Monoclonal Antibody
Related Products
Agarose Substrate
- High cross-linked agarose
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
Ni Affinity Resin
- Ni NTA/NTA+ Resin
- Ni IDA Resin
- Ni TED Resin
- Benzamidine Affinity Media
mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range