Protein A Affinity Resin
Sepromax® A40 Plus
Protein A affinity resin Sepromax® A40 plus designed for the isolation and purification of monoclonal antibodies. Compared with traditional agarose media, Sepromax® A40 plus has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of the ligand. 0.1-0.5M NaOH can use for clean-in-Place (CIP).
Sepromax® A40 plus is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS/DVB microsphere substrate. rProtein A has better alkali-resisting that ensures excellent stability in high pH conditions. With GLK® hydrophilic treatment and binding technology, PS/DVB surface removes non-specific binding with right combination of rProtein A ligand. Hence, Sepromax® A40 plus is extremely useful for isolating monoclonal antibodies.
Main Characteristics
| Parameter | Description |
|---|---|
| Support Matrix | Rigid, porous, mono-dispersed PS-DVB particles |
| Particle Size | 40 μm |
| Dynamic Binding Capacity (max) | 60 mg human IgG/mL (10% Penetrating Frontier Analysis) |
| Recommended Working Flow Rate | 300 – 700 cm/h |
| Maximum Operating Pressure | 1500 psi (100 bar or 10 MPa) |
| PH Stability | 2 – 10 (long term) 2-13 (CIP) |
| Cleaning Agent | 0.1-0.5 M NaOH |
| Storage | 20％ Ethanol/water at 2 – 8℃ |
Advantages Of Protein A Affinity Resin
- High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing.
- Outstanding high dynamic binding capacity at low residence time reduces process time and amount of medium used.
- Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP.
- Low ligand leakage ensures a long lifetime.
- High price-performance ratio.
Performance Testing
1. Particle images
2. Dynamic Binding Capacity (DBC)
| Test Condition | |
|---|---|
| System | AKTA® Purifier10 |
| Initial Buffer | 20 mM PB，0.15 M NaCl，pH 7.4 |
| Elution Buffer | 0.1 M Gly-HCl，pH 2.5 |
| CIP solution | 0.5 M NaOH |
| Sample | 1.0 mg/ml hIgG |
3. Protein A Ligand Leakage Test (ELISA)
Samples: 20mg hlgG/ml-resin, 9mg mAb/ml-resin
ELISA test: Cygnus F400 kit
4. Competitive tests of competitive product
Protein A Affinity Resin Application
1.Fc Protein
2.Monoclonal Antibody
Related Products
Agarose Substrate
- High cross-linked agarose
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
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mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range