Protein A Affinity Resin

Sepromax® A40 Plus

Protein A affinity resin Sepromax® A40 plus designed for the isolation and purification of monoclonal antibodies. Compared with traditional agarose media, Sepromax® A40 plus has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of the ligand. 0.1-0.5M NaOH can use for clean-in-Place (CIP).

Sepromax® A40 plus is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS/DVB microsphere substrate. rProtein A has better alkali-resisting that ensures excellent stability in high pH conditions. With GLK® hydrophilic treatment and binding technology, PS/DVB surface removes non-specific binding with right combination of rProtein A ligand. Hence, Sepromax® A40 plus is extremely useful for isolating monoclonal antibodies.

GALAK Products Catalog

Main Characteristics

Parameter	Description
Support Matrix	Rigid, porous, mono-dispersed PS-DVB particles
Particle Size	40 μm
Dynamic Binding Capacity (max)	60 mg human IgG/mL (10% Penetrating Frontier Analysis)
Recommended Working Flow Rate	300 – 700 cm/h
Maximum Operating Pressure	1500 psi (100 bar or 10 MPa)
PH Stability	2 – 10 (long term) 2-13 (CIP)
Cleaning Agent	0.1-0.5 M NaOH
Storage	20％ Ethanol/water at 2 – 8℃

Advantages Of Protein A Affinity Resin

High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing.
Outstanding high dynamic binding capacity at low residence time reduces process time and amount of medium used.
Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP.
Low ligand leakage ensures a long lifetime.
High price-performance ratio.