Protein A/G Magnetic Beads

Protein A/G Magnetic Beads products are magnetic beads designed for high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from various species.

Protein A/G Magnetic Beads are intended for research only, and should not be used in any clinical or in vitro procedures for diagnostic purposes.

rProtein-A/G-Magarose-Beads

Protein A/G Magnetic Beads Parameters

SubstrateMagnetic agarose microspheres
LigandsRecombinant protein A/G
Combined ability (/ml magnetic beads)>10mg hlgG
Particle size(μm)30-100
Storage buffer1XPBS containing 20% ethanol
VolumeSuspend in protection solution, 20% content
Storage temperature2°C-8°C

Protein A/G Magnetic Beads Introduction

Agarose base magnetic microspheres series products (Magarose beads) have the characteristics of superparamagnetism, rapid magnetic responsiveness, abundant hydroxyl functional groups, and relatively concentrated particle size, which are important carrier tools in medical and molecular biology research.

GALAK Chromatography adopts unique production technology to prepare magnetic agarose microspheres with a particle size distribution of 30-100 × m, with moderate particle size, which is more suitable for biological examination and purification experiments.

Protein A Agarose Beads transfer rProtein A high-density directional packets to the surface of superparamagnetic microspheres. The product has higher antibody binding capacity and lower non-specific Protein adsorption rate. Under more homogeneous elution conditions, antibodies with purity greater than 90% can be separated from serum samples. Protein A is a cell wall protein isolated from Staphylococcus aureus that binds mammalian IgG primarily through Fc fragments, but not to dog lgG and not to human lgM, lgD, and IgA. Protein A and protein G have different binding ability to immunoglobulin from different sources and subclasses. Natural Protein A has five IgG-binding regions and some regions with unknown functions, while recombinant Protein A contains only five lgG binding regions without binding sites to albumin and cell surface, reducing non-specific adsorption.

rProtein A magnetic beads is a micron magnetic microsphere with a large specific surface area, which can greatly shorten the time required for antibody adsorption. The product performance is shown in table 1. This product is suitable for antibody purification in plasma, ascites, tissue culture supernatant, and other samples, as well as antibody fixation and other related studies. Users can select the category of magnetic beads according to the species source and subtype of the target antibody. The compatibility of Protein A, Protein G, and Protein A /G with different antibodies is shown in the attached table.

Binding Ability of Protein A, Protein G, and Protein A/G

SpeciesSubtypeProtein AProtein GProtein A/G
HumanIgAvariable_++
 IgD___
 IgE___
 IgG1++++++++++++
 IgG2++++++++++++
 IgG3_++++++++
 IgG4++++++++++++
 IgMvariable_++
Avian egg yolkIgY___
Cow ++++++++++
Dog ++++++++++
Goat _++++++++
Guinea pigIgG1++++++++++
 IgG2++++++++++
Hamster +++ 
HorseTotal IgG++++++++++
Koala _+ 
Liama _+ 
Monkey(rhesus) ++++++++++++
MouseIgG1+++++++
 IgG2a++++++++++++
 IgG2b+++++++++
 IgG3++++++++
 IgMvariable__
Pig ++++++++++
RabbitTotal IgG+++++++++++
RatIgG1_+++
 IgG2a_++++++++
 IgG2b_++++
 IgG3+++++
SheetTotal IgG+/-++++

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