One-step Live Attenuated Rabies Virus Vaccine Purification

Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). In up to 99% of cases, domestic dogs are responsible for rabies virus transmission to humans. Yet, rabies can affect both domestic and wild animals. It is spread to people through bites or scratches, usually via saliva. Without rabies vaccine, the fatality rate is nearly 100% fatality rate when humans and pets are infected. (World Health Organization). Rabies vaccines are the most effective method to protect human and pets when they are exposed.

Compare with traditional rabies vaccine purification (membrane separation and size exclusion), GALAK VirCap® AF media can purify the live attenuated rabies virus just in one step with better virus protein recovery and purity.

rabies virus

Rabies Vaccine Purification Tests

Result

  • Recovery > 80%
  • Purity > 90%
  • Removal rate of impurities > 99%

Test Condition:

Column:  VirCap® AF 1ml prepacked column

Mobile phase A: 10mM PB pH7.4

Mobile phase B: 10mM PB pH7.4 2M NaCl

Flow Rate: 0.5ml/min

Testing: UV 280nm

Sample: Clarified virus medium

rabies vaccine purification chromatogram

Using HPSEC analysis for the tests of sample solution, percolation fluid and collection fluid, and no peak of target in breakthough at RT=10min. VirCap® AF media elutes virus particles by changing the ion strength in mobile phase. Both the purity and concentration of the virus improves significantly.

Parallel tests of HPSEC analysis for target peak collection is also done among competitors.

rabies vaccine purification chromatogram
rabies vaccine purification chromatogram

CIP and Clearance

Frequency of Clean-in-Place (CIP) depends on properties and conditions of solvent.

For the capture step, we suppose at least 30 minutes of 1 M NaOH countercurrent CIP process after each running.

To reduce microbial contamination in the prepacked column, clean with 0.5 to 1.0 M NaOH, 1 h contact time is recommended.

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