SEC Column

GALAK SEC is a family of high-performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of the analyte. The column technology involves the creation of a neutral hydrophilic layer on the surface of specially designed high-strength, monodispersed, silica particles followed by a well-established production process. Therefore, GALAK SEC columns have abroad application range in pharmaceutical, biopharmaceutical, and academic research.

SEC column process

Features

  • High column efficiency, high resolution;
  • Minimal undesired interactions between the stationary phase and analytes, resulting in good peak shape and recovery;
  • High physical strength for better column lifetime;
  • The broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.

Types

SEC-150 - designed for separating peptides, glycans, small oligos, small proteins.
SEC-300 - designed for mAb aggregate determination.
SEC-500 - designed for separating larger proteins and oligos.

 SEC-150SEC-300SEC-500
LigandDiol
SubstrateMonodisperse High-pure Silica Particle
Particle Size5um
Pore Size150A300A500A
pH Range 2-8
Temperature<40℃
Pressure6000psi
Ligand Range

(PEG)

200-15,0001.000-100,0005,000-200,000
Ligand Range

(Glucan)

1,000-50,0005,000-150,00020,000-500,000
Ligand Range

(Globular Protein)

5,000-150,00010,000-1,000,00020,000-2,000,000
SEC Column comparasion

Column Black: SEC-150, 5μm

Column Blue: SEC-300, 5μm

Column Red: SEC-500, 5μm

Dimension: 4.6×300mm

Mobile phase: 150 mM Phosphate Buffered Saline (pH 6.8)

Flow rate: 0.35 mL/min

Temperature: 30 ℃

Injection: 5μL

Detection: UV 280 nm

Peaks:

1. Thyroglobulin (0.5mg/mL) -669,000Da

2. Conalbumin (1mg/mL) -75,000Da

3. Ribonuclease A (1mg/mL) -13,700Da

4. Uracil (0.1mg/mL) ‒112Da

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